Stable-isotope reductive dimethylation, a cost-effective, simple, robust, reliable and easy-to- multiplex labelling method, is widely applied to quantitative proteomics using liquid chromatography-mass spectrometry. This review focuses on biological applications of stable-isotope dimethyl labelling for a large-scale comparative analysis of protein expression and post-translational modifications based on its unique properties of the labelling chemistry. Some other applications of the labelling method for sample preparation and mass spectrometry-based protein identification and characterization are also summarized.
This article is part of the themed issue ‘Quantitative mass spectrometry’.
One contribution of 19 to a theme issue ‘Quantitative mass spectrometry’.
- Accepted May 6, 2016.
- © 2016 The Author(s)
Published by the Royal Society. All rights reserved.